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sp 2001 bca protein assay kit takara  (Vector Laboratories)


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    Vector Laboratories sp 2001 bca protein assay kit takara
    Sp 2001 Bca Protein Assay Kit Takara, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 11535 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sp+2001+bca+protein+assay+kit+takara/Avidin%2FBiotin+Blocking+Kit/pm35263589-248-161-159
    Average 96 stars, based on 11535 article reviews
    sp 2001 bca protein assay kit takara - by Bioz Stars, 2026-09
    96/100 stars

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    Enzyme-linked Immunosorbent Assay:

    Article Title: Nociceptor-derived Reg3γ prevents endotoxic death by targeting kynurenine pathway in microglia.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER CD11c MicroBeads Myltenyi Biotec Cat# 130-108-338 Mouse TNF-a ELISA kit R&D systems Cat# DY410-05 Mouse IL-6 ELISA kit R&D systems Cat# M6000B Mouse IL-1b ELISA kit R&D systems Cat# DY401 Mouse IL-12 ELISA kit R&D systems Cat# M1270 Mouse Alanine Aminotransferase ELISA Kit MyBiosource Cat# MBS264717 Creatinine Colorimetric Assay Kit Cayman Cat# 700460 Mouse Troponin I, cardiac muscle ELISA Kit Signalway Antibody Cat# EK1821 Mouse Reg3g ELISA kit LSBio Cat# LS-F6890 ATPlite PerkinElmer Cat# 6016493 Glucose 6 Phosphate Assay Kit abcam Cat# ab107923 Phosphoenolpyruvate Assay Kit Abnova Cat# KA3745 Kynurenine / Tryptophan ratio ELISA pack ImmuSmol Cat# ISE-2227 Kynurenine ELISA Kit BioVision Cat# E4629 Quinolinic acid ELISA ImmuSmol Cat# IS I-0100 Phospho-c-Src + Total In-Cell ELISA kit abcam Cat# ab207461 Rac1 G-LISA Activation Assay Kit Cytoskeleton Cat# BK128 ReverTra Ace Toyobo Cat# FSQ-201 Taqman Ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 HMT internal standards Human Metabolome Technologies Cat# H3304-1002 Avidin/biotin blocking kit Vector Cat# SP-2001 BCA protein assay kit TaKaRa Cat# T9300A Western Lightning Plus-ECL reagents Parkin Elmer Cat# NEL103001EA Low Salt Immune Complex Wash Buffer Millipore Cat# 20154 High Salt Immune Complex Wash Buffer Millipore Cat# 20155 LiCl Immune Complex Wash Buffer Millipore Cat# 20156 RNAeasy Mini Kit Qiagen Cat# 74104 Deposited data Microarray data of brain GEO database https://www.ncbi. nlm.gov/geo/ Accession number: GEO: GSE173216 Experimental models: Cell lines Cell line: Ra2 COSMO BIO COS-NMG-RA2C Cell line: N2A ATCC ATCC CCL-131 Cell line: RAW264.7 ATCC ATCC TIB-72 Experimental models: Organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Nav1.8-Cre EMMA Stock ID: EM:04582 Mouse: Rosa26-DTA Jackson Laboratory Stock number: 010527 Mouse: Bcl10-/- Provided by S. Akira Ruefli-Brasse et al. (2003) Mouse: TLR4-/- Purchased from OrientalBioService Hoshino et al. (1999) Mouse: TRPA1-/- Provided by D. Julius Bautista et al. (2006) Mouse: FLPo-Cre Provided by M. Ikawa Yamazaki et al. (2016) Mouse: Reg3gtm1a(KOMP) KOMP repository Strain ID: Reg3 g tm1a (KOMP) Mouse: Villin-Cre Provided by S. Akira el Marjou et al. (2004) Oligonucleotides Genotyping DNA primers (Table S1) This paper N/A siRNA Extl3 MSS225704 UCGUUGUCCU CUGACGUCUGGCUUU Life Technologies Cat# 10620318 siRNA XIAP MSS202115 GCUCACGG AUUGGAAGCCAAGUGAA Life Technologies Cat# 10620318 (Continued on next page) Cell Reports 38, 110462, March 8, 2022 e2

    Colorimetric Assay:

    Article Title: Nociceptor-derived Reg3γ prevents endotoxic death by targeting kynurenine pathway in microglia.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER CD11c MicroBeads Myltenyi Biotec Cat# 130-108-338 Mouse TNF-a ELISA kit R&D systems Cat# DY410-05 Mouse IL-6 ELISA kit R&D systems Cat# M6000B Mouse IL-1b ELISA kit R&D systems Cat# DY401 Mouse IL-12 ELISA kit R&D systems Cat# M1270 Mouse Alanine Aminotransferase ELISA Kit MyBiosource Cat# MBS264717 Creatinine Colorimetric Assay Kit Cayman Cat# 700460 Mouse Troponin I, cardiac muscle ELISA Kit Signalway Antibody Cat# EK1821 Mouse Reg3g ELISA kit LSBio Cat# LS-F6890 ATPlite PerkinElmer Cat# 6016493 Glucose 6 Phosphate Assay Kit abcam Cat# ab107923 Phosphoenolpyruvate Assay Kit Abnova Cat# KA3745 Kynurenine / Tryptophan ratio ELISA pack ImmuSmol Cat# ISE-2227 Kynurenine ELISA Kit BioVision Cat# E4629 Quinolinic acid ELISA ImmuSmol Cat# IS I-0100 Phospho-c-Src + Total In-Cell ELISA kit abcam Cat# ab207461 Rac1 G-LISA Activation Assay Kit Cytoskeleton Cat# BK128 ReverTra Ace Toyobo Cat# FSQ-201 Taqman Ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 HMT internal standards Human Metabolome Technologies Cat# H3304-1002 Avidin/biotin blocking kit Vector Cat# SP-2001 BCA protein assay kit TaKaRa Cat# T9300A Western Lightning Plus-ECL reagents Parkin Elmer Cat# NEL103001EA Low Salt Immune Complex Wash Buffer Millipore Cat# 20154 High Salt Immune Complex Wash Buffer Millipore Cat# 20155 LiCl Immune Complex Wash Buffer Millipore Cat# 20156 RNAeasy Mini Kit Qiagen Cat# 74104 Deposited data Microarray data of brain GEO database https://www.ncbi. nlm.gov/geo/ Accession number: GEO: GSE173216 Experimental models: Cell lines Cell line: Ra2 COSMO BIO COS-NMG-RA2C Cell line: N2A ATCC ATCC CCL-131 Cell line: RAW264.7 ATCC ATCC TIB-72 Experimental models: Organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Nav1.8-Cre EMMA Stock ID: EM:04582 Mouse: Rosa26-DTA Jackson Laboratory Stock number: 010527 Mouse: Bcl10-/- Provided by S. Akira Ruefli-Brasse et al. (2003) Mouse: TLR4-/- Purchased from OrientalBioService Hoshino et al. (1999) Mouse: TRPA1-/- Provided by D. Julius Bautista et al. (2006) Mouse: FLPo-Cre Provided by M. Ikawa Yamazaki et al. (2016) Mouse: Reg3gtm1a(KOMP) KOMP repository Strain ID: Reg3 g tm1a (KOMP) Mouse: Villin-Cre Provided by S. Akira el Marjou et al. (2004) Oligonucleotides Genotyping DNA primers (Table S1) This paper N/A siRNA Extl3 MSS225704 UCGUUGUCCU CUGACGUCUGGCUUU Life Technologies Cat# 10620318 siRNA XIAP MSS202115 GCUCACGG AUUGGAAGCCAAGUGAA Life Technologies Cat# 10620318 (Continued on next page) Cell Reports 38, 110462, March 8, 2022 e2

    In-Cell ELISA:

    Article Title: Nociceptor-derived Reg3γ prevents endotoxic death by targeting kynurenine pathway in microglia.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER CD11c MicroBeads Myltenyi Biotec Cat# 130-108-338 Mouse TNF-a ELISA kit R&D systems Cat# DY410-05 Mouse IL-6 ELISA kit R&D systems Cat# M6000B Mouse IL-1b ELISA kit R&D systems Cat# DY401 Mouse IL-12 ELISA kit R&D systems Cat# M1270 Mouse Alanine Aminotransferase ELISA Kit MyBiosource Cat# MBS264717 Creatinine Colorimetric Assay Kit Cayman Cat# 700460 Mouse Troponin I, cardiac muscle ELISA Kit Signalway Antibody Cat# EK1821 Mouse Reg3g ELISA kit LSBio Cat# LS-F6890 ATPlite PerkinElmer Cat# 6016493 Glucose 6 Phosphate Assay Kit abcam Cat# ab107923 Phosphoenolpyruvate Assay Kit Abnova Cat# KA3745 Kynurenine / Tryptophan ratio ELISA pack ImmuSmol Cat# ISE-2227 Kynurenine ELISA Kit BioVision Cat# E4629 Quinolinic acid ELISA ImmuSmol Cat# IS I-0100 Phospho-c-Src + Total In-Cell ELISA kit abcam Cat# ab207461 Rac1 G-LISA Activation Assay Kit Cytoskeleton Cat# BK128 ReverTra Ace Toyobo Cat# FSQ-201 Taqman Ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 HMT internal standards Human Metabolome Technologies Cat# H3304-1002 Avidin/biotin blocking kit Vector Cat# SP-2001 BCA protein assay kit TaKaRa Cat# T9300A Western Lightning Plus-ECL reagents Parkin Elmer Cat# NEL103001EA Low Salt Immune Complex Wash Buffer Millipore Cat# 20154 High Salt Immune Complex Wash Buffer Millipore Cat# 20155 LiCl Immune Complex Wash Buffer Millipore Cat# 20156 RNAeasy Mini Kit Qiagen Cat# 74104 Deposited data Microarray data of brain GEO database https://www.ncbi. nlm.gov/geo/ Accession number: GEO: GSE173216 Experimental models: Cell lines Cell line: Ra2 COSMO BIO COS-NMG-RA2C Cell line: N2A ATCC ATCC CCL-131 Cell line: RAW264.7 ATCC ATCC TIB-72 Experimental models: Organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Nav1.8-Cre EMMA Stock ID: EM:04582 Mouse: Rosa26-DTA Jackson Laboratory Stock number: 010527 Mouse: Bcl10-/- Provided by S. Akira Ruefli-Brasse et al. (2003) Mouse: TLR4-/- Purchased from OrientalBioService Hoshino et al. (1999) Mouse: TRPA1-/- Provided by D. Julius Bautista et al. (2006) Mouse: FLPo-Cre Provided by M. Ikawa Yamazaki et al. (2016) Mouse: Reg3gtm1a(KOMP) KOMP repository Strain ID: Reg3 g tm1a (KOMP) Mouse: Villin-Cre Provided by S. Akira el Marjou et al. (2004) Oligonucleotides Genotyping DNA primers (Table S1) This paper N/A siRNA Extl3 MSS225704 UCGUUGUCCU CUGACGUCUGGCUUU Life Technologies Cat# 10620318 siRNA XIAP MSS202115 GCUCACGG AUUGGAAGCCAAGUGAA Life Technologies Cat# 10620318 (Continued on next page) Cell Reports 38, 110462, March 8, 2022 e2

    Activation Assay:

    Article Title: Nociceptor-derived Reg3γ prevents endotoxic death by targeting kynurenine pathway in microglia.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER CD11c MicroBeads Myltenyi Biotec Cat# 130-108-338 Mouse TNF-a ELISA kit R&D systems Cat# DY410-05 Mouse IL-6 ELISA kit R&D systems Cat# M6000B Mouse IL-1b ELISA kit R&D systems Cat# DY401 Mouse IL-12 ELISA kit R&D systems Cat# M1270 Mouse Alanine Aminotransferase ELISA Kit MyBiosource Cat# MBS264717 Creatinine Colorimetric Assay Kit Cayman Cat# 700460 Mouse Troponin I, cardiac muscle ELISA Kit Signalway Antibody Cat# EK1821 Mouse Reg3g ELISA kit LSBio Cat# LS-F6890 ATPlite PerkinElmer Cat# 6016493 Glucose 6 Phosphate Assay Kit abcam Cat# ab107923 Phosphoenolpyruvate Assay Kit Abnova Cat# KA3745 Kynurenine / Tryptophan ratio ELISA pack ImmuSmol Cat# ISE-2227 Kynurenine ELISA Kit BioVision Cat# E4629 Quinolinic acid ELISA ImmuSmol Cat# IS I-0100 Phospho-c-Src + Total In-Cell ELISA kit abcam Cat# ab207461 Rac1 G-LISA Activation Assay Kit Cytoskeleton Cat# BK128 ReverTra Ace Toyobo Cat# FSQ-201 Taqman Ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 HMT internal standards Human Metabolome Technologies Cat# H3304-1002 Avidin/biotin blocking kit Vector Cat# SP-2001 BCA protein assay kit TaKaRa Cat# T9300A Western Lightning Plus-ECL reagents Parkin Elmer Cat# NEL103001EA Low Salt Immune Complex Wash Buffer Millipore Cat# 20154 High Salt Immune Complex Wash Buffer Millipore Cat# 20155 LiCl Immune Complex Wash Buffer Millipore Cat# 20156 RNAeasy Mini Kit Qiagen Cat# 74104 Deposited data Microarray data of brain GEO database https://www.ncbi. nlm.gov/geo/ Accession number: GEO: GSE173216 Experimental models: Cell lines Cell line: Ra2 COSMO BIO COS-NMG-RA2C Cell line: N2A ATCC ATCC CCL-131 Cell line: RAW264.7 ATCC ATCC TIB-72 Experimental models: Organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Nav1.8-Cre EMMA Stock ID: EM:04582 Mouse: Rosa26-DTA Jackson Laboratory Stock number: 010527 Mouse: Bcl10-/- Provided by S. Akira Ruefli-Brasse et al. (2003) Mouse: TLR4-/- Purchased from OrientalBioService Hoshino et al. (1999) Mouse: TRPA1-/- Provided by D. Julius Bautista et al. (2006) Mouse: FLPo-Cre Provided by M. Ikawa Yamazaki et al. (2016) Mouse: Reg3gtm1a(KOMP) KOMP repository Strain ID: Reg3 g tm1a (KOMP) Mouse: Villin-Cre Provided by S. Akira el Marjou et al. (2004) Oligonucleotides Genotyping DNA primers (Table S1) This paper N/A siRNA Extl3 MSS225704 UCGUUGUCCU CUGACGUCUGGCUUU Life Technologies Cat# 10620318 siRNA XIAP MSS202115 GCUCACGG AUUGGAAGCCAAGUGAA Life Technologies Cat# 10620318 (Continued on next page) Cell Reports 38, 110462, March 8, 2022 e2

    Control:

    Article Title: Nociceptor-derived Reg3γ prevents endotoxic death by targeting kynurenine pathway in microglia.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER CD11c MicroBeads Myltenyi Biotec Cat# 130-108-338 Mouse TNF-a ELISA kit R&D systems Cat# DY410-05 Mouse IL-6 ELISA kit R&D systems Cat# M6000B Mouse IL-1b ELISA kit R&D systems Cat# DY401 Mouse IL-12 ELISA kit R&D systems Cat# M1270 Mouse Alanine Aminotransferase ELISA Kit MyBiosource Cat# MBS264717 Creatinine Colorimetric Assay Kit Cayman Cat# 700460 Mouse Troponin I, cardiac muscle ELISA Kit Signalway Antibody Cat# EK1821 Mouse Reg3g ELISA kit LSBio Cat# LS-F6890 ATPlite PerkinElmer Cat# 6016493 Glucose 6 Phosphate Assay Kit abcam Cat# ab107923 Phosphoenolpyruvate Assay Kit Abnova Cat# KA3745 Kynurenine / Tryptophan ratio ELISA pack ImmuSmol Cat# ISE-2227 Kynurenine ELISA Kit BioVision Cat# E4629 Quinolinic acid ELISA ImmuSmol Cat# IS I-0100 Phospho-c-Src + Total In-Cell ELISA kit abcam Cat# ab207461 Rac1 G-LISA Activation Assay Kit Cytoskeleton Cat# BK128 ReverTra Ace Toyobo Cat# FSQ-201 Taqman Ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 HMT internal standards Human Metabolome Technologies Cat# H3304-1002 Avidin/biotin blocking kit Vector Cat# SP-2001 BCA protein assay kit TaKaRa Cat# T9300A Western Lightning Plus-ECL reagents Parkin Elmer Cat# NEL103001EA Low Salt Immune Complex Wash Buffer Millipore Cat# 20154 High Salt Immune Complex Wash Buffer Millipore Cat# 20155 LiCl Immune Complex Wash Buffer Millipore Cat# 20156 RNAeasy Mini Kit Qiagen Cat# 74104 Deposited data Microarray data of brain GEO database https://www.ncbi. nlm.gov/geo/ Accession number: GEO: GSE173216 Experimental models: Cell lines Cell line: Ra2 COSMO BIO COS-NMG-RA2C Cell line: N2A ATCC ATCC CCL-131 Cell line: RAW264.7 ATCC ATCC TIB-72 Experimental models: Organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Nav1.8-Cre EMMA Stock ID: EM:04582 Mouse: Rosa26-DTA Jackson Laboratory Stock number: 010527 Mouse: Bcl10-/- Provided by S. Akira Ruefli-Brasse et al. (2003) Mouse: TLR4-/- Purchased from OrientalBioService Hoshino et al. (1999) Mouse: TRPA1-/- Provided by D. Julius Bautista et al. (2006) Mouse: FLPo-Cre Provided by M. Ikawa Yamazaki et al. (2016) Mouse: Reg3gtm1a(KOMP) KOMP repository Strain ID: Reg3 g tm1a (KOMP) Mouse: Villin-Cre Provided by S. Akira el Marjou et al. (2004) Oligonucleotides Genotyping DNA primers (Table S1) This paper N/A siRNA Extl3 MSS225704 UCGUUGUCCU CUGACGUCUGGCUUU Life Technologies Cat# 10620318 siRNA XIAP MSS202115 GCUCACGG AUUGGAAGCCAAGUGAA Life Technologies Cat# 10620318 (Continued on next page) Cell Reports 38, 110462, March 8, 2022 e2

    HMT Assay:

    Article Title: Nociceptor-derived Reg3γ prevents endotoxic death by targeting kynurenine pathway in microglia.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER CD11c MicroBeads Myltenyi Biotec Cat# 130-108-338 Mouse TNF-a ELISA kit R&D systems Cat# DY410-05 Mouse IL-6 ELISA kit R&D systems Cat# M6000B Mouse IL-1b ELISA kit R&D systems Cat# DY401 Mouse IL-12 ELISA kit R&D systems Cat# M1270 Mouse Alanine Aminotransferase ELISA Kit MyBiosource Cat# MBS264717 Creatinine Colorimetric Assay Kit Cayman Cat# 700460 Mouse Troponin I, cardiac muscle ELISA Kit Signalway Antibody Cat# EK1821 Mouse Reg3g ELISA kit LSBio Cat# LS-F6890 ATPlite PerkinElmer Cat# 6016493 Glucose 6 Phosphate Assay Kit abcam Cat# ab107923 Phosphoenolpyruvate Assay Kit Abnova Cat# KA3745 Kynurenine / Tryptophan ratio ELISA pack ImmuSmol Cat# ISE-2227 Kynurenine ELISA Kit BioVision Cat# E4629 Quinolinic acid ELISA ImmuSmol Cat# IS I-0100 Phospho-c-Src + Total In-Cell ELISA kit abcam Cat# ab207461 Rac1 G-LISA Activation Assay Kit Cytoskeleton Cat# BK128 ReverTra Ace Toyobo Cat# FSQ-201 Taqman Ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 HMT internal standards Human Metabolome Technologies Cat# H3304-1002 Avidin/biotin blocking kit Vector Cat# SP-2001 BCA protein assay kit TaKaRa Cat# T9300A Western Lightning Plus-ECL reagents Parkin Elmer Cat# NEL103001EA Low Salt Immune Complex Wash Buffer Millipore Cat# 20154 High Salt Immune Complex Wash Buffer Millipore Cat# 20155 LiCl Immune Complex Wash Buffer Millipore Cat# 20156 RNAeasy Mini Kit Qiagen Cat# 74104 Deposited data Microarray data of brain GEO database https://www.ncbi. nlm.gov/geo/ Accession number: GEO: GSE173216 Experimental models: Cell lines Cell line: Ra2 COSMO BIO COS-NMG-RA2C Cell line: N2A ATCC ATCC CCL-131 Cell line: RAW264.7 ATCC ATCC TIB-72 Experimental models: Organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Nav1.8-Cre EMMA Stock ID: EM:04582 Mouse: Rosa26-DTA Jackson Laboratory Stock number: 010527 Mouse: Bcl10-/- Provided by S. Akira Ruefli-Brasse et al. (2003) Mouse: TLR4-/- Purchased from OrientalBioService Hoshino et al. (1999) Mouse: TRPA1-/- Provided by D. Julius Bautista et al. (2006) Mouse: FLPo-Cre Provided by M. Ikawa Yamazaki et al. (2016) Mouse: Reg3gtm1a(KOMP) KOMP repository Strain ID: Reg3 g tm1a (KOMP) Mouse: Villin-Cre Provided by S. Akira el Marjou et al. (2004) Oligonucleotides Genotyping DNA primers (Table S1) This paper N/A siRNA Extl3 MSS225704 UCGUUGUCCU CUGACGUCUGGCUUU Life Technologies Cat# 10620318 siRNA XIAP MSS202115 GCUCACGG AUUGGAAGCCAAGUGAA Life Technologies Cat# 10620318 (Continued on next page) Cell Reports 38, 110462, March 8, 2022 e2

    Avidin-Biotin Assay:

    Article Title: Nociceptor-derived Reg3γ prevents endotoxic death by targeting kynurenine pathway in microglia.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER CD11c MicroBeads Myltenyi Biotec Cat# 130-108-338 Mouse TNF-a ELISA kit R&D systems Cat# DY410-05 Mouse IL-6 ELISA kit R&D systems Cat# M6000B Mouse IL-1b ELISA kit R&D systems Cat# DY401 Mouse IL-12 ELISA kit R&D systems Cat# M1270 Mouse Alanine Aminotransferase ELISA Kit MyBiosource Cat# MBS264717 Creatinine Colorimetric Assay Kit Cayman Cat# 700460 Mouse Troponin I, cardiac muscle ELISA Kit Signalway Antibody Cat# EK1821 Mouse Reg3g ELISA kit LSBio Cat# LS-F6890 ATPlite PerkinElmer Cat# 6016493 Glucose 6 Phosphate Assay Kit abcam Cat# ab107923 Phosphoenolpyruvate Assay Kit Abnova Cat# KA3745 Kynurenine / Tryptophan ratio ELISA pack ImmuSmol Cat# ISE-2227 Kynurenine ELISA Kit BioVision Cat# E4629 Quinolinic acid ELISA ImmuSmol Cat# IS I-0100 Phospho-c-Src + Total In-Cell ELISA kit abcam Cat# ab207461 Rac1 G-LISA Activation Assay Kit Cytoskeleton Cat# BK128 ReverTra Ace Toyobo Cat# FSQ-201 Taqman Ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 HMT internal standards Human Metabolome Technologies Cat# H3304-1002 Avidin/biotin blocking kit Vector Cat# SP-2001 BCA protein assay kit TaKaRa Cat# T9300A Western Lightning Plus-ECL reagents Parkin Elmer Cat# NEL103001EA Low Salt Immune Complex Wash Buffer Millipore Cat# 20154 High Salt Immune Complex Wash Buffer Millipore Cat# 20155 LiCl Immune Complex Wash Buffer Millipore Cat# 20156 RNAeasy Mini Kit Qiagen Cat# 74104 Deposited data Microarray data of brain GEO database https://www.ncbi. nlm.gov/geo/ Accession number: GEO: GSE173216 Experimental models: Cell lines Cell line: Ra2 COSMO BIO COS-NMG-RA2C Cell line: N2A ATCC ATCC CCL-131 Cell line: RAW264.7 ATCC ATCC TIB-72 Experimental models: Organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Nav1.8-Cre EMMA Stock ID: EM:04582 Mouse: Rosa26-DTA Jackson Laboratory Stock number: 010527 Mouse: Bcl10-/- Provided by S. Akira Ruefli-Brasse et al. (2003) Mouse: TLR4-/- Purchased from OrientalBioService Hoshino et al. (1999) Mouse: TRPA1-/- Provided by D. Julius Bautista et al. (2006) Mouse: FLPo-Cre Provided by M. Ikawa Yamazaki et al. (2016) Mouse: Reg3gtm1a(KOMP) KOMP repository Strain ID: Reg3 g tm1a (KOMP) Mouse: Villin-Cre Provided by S. Akira el Marjou et al. (2004) Oligonucleotides Genotyping DNA primers (Table S1) This paper N/A siRNA Extl3 MSS225704 UCGUUGUCCU CUGACGUCUGGCUUU Life Technologies Cat# 10620318 siRNA XIAP MSS202115 GCUCACGG AUUGGAAGCCAAGUGAA Life Technologies Cat# 10620318 (Continued on next page) Cell Reports 38, 110462, March 8, 2022 e2

    Blocking Assay:

    Article Title: Nociceptor-derived Reg3γ prevents endotoxic death by targeting kynurenine pathway in microglia.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER CD11c MicroBeads Myltenyi Biotec Cat# 130-108-338 Mouse TNF-a ELISA kit R&D systems Cat# DY410-05 Mouse IL-6 ELISA kit R&D systems Cat# M6000B Mouse IL-1b ELISA kit R&D systems Cat# DY401 Mouse IL-12 ELISA kit R&D systems Cat# M1270 Mouse Alanine Aminotransferase ELISA Kit MyBiosource Cat# MBS264717 Creatinine Colorimetric Assay Kit Cayman Cat# 700460 Mouse Troponin I, cardiac muscle ELISA Kit Signalway Antibody Cat# EK1821 Mouse Reg3g ELISA kit LSBio Cat# LS-F6890 ATPlite PerkinElmer Cat# 6016493 Glucose 6 Phosphate Assay Kit abcam Cat# ab107923 Phosphoenolpyruvate Assay Kit Abnova Cat# KA3745 Kynurenine / Tryptophan ratio ELISA pack ImmuSmol Cat# ISE-2227 Kynurenine ELISA Kit BioVision Cat# E4629 Quinolinic acid ELISA ImmuSmol Cat# IS I-0100 Phospho-c-Src + Total In-Cell ELISA kit abcam Cat# ab207461 Rac1 G-LISA Activation Assay Kit Cytoskeleton Cat# BK128 ReverTra Ace Toyobo Cat# FSQ-201 Taqman Ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 HMT internal standards Human Metabolome Technologies Cat# H3304-1002 Avidin/biotin blocking kit Vector Cat# SP-2001 BCA protein assay kit TaKaRa Cat# T9300A Western Lightning Plus-ECL reagents Parkin Elmer Cat# NEL103001EA Low Salt Immune Complex Wash Buffer Millipore Cat# 20154 High Salt Immune Complex Wash Buffer Millipore Cat# 20155 LiCl Immune Complex Wash Buffer Millipore Cat# 20156 RNAeasy Mini Kit Qiagen Cat# 74104 Deposited data Microarray data of brain GEO database https://www.ncbi. nlm.gov/geo/ Accession number: GEO: GSE173216 Experimental models: Cell lines Cell line: Ra2 COSMO BIO COS-NMG-RA2C Cell line: N2A ATCC ATCC CCL-131 Cell line: RAW264.7 ATCC ATCC TIB-72 Experimental models: Organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Nav1.8-Cre EMMA Stock ID: EM:04582 Mouse: Rosa26-DTA Jackson Laboratory Stock number: 010527 Mouse: Bcl10-/- Provided by S. Akira Ruefli-Brasse et al. (2003) Mouse: TLR4-/- Purchased from OrientalBioService Hoshino et al. (1999) Mouse: TRPA1-/- Provided by D. Julius Bautista et al. (2006) Mouse: FLPo-Cre Provided by M. Ikawa Yamazaki et al. (2016) Mouse: Reg3gtm1a(KOMP) KOMP repository Strain ID: Reg3 g tm1a (KOMP) Mouse: Villin-Cre Provided by S. Akira el Marjou et al. (2004) Oligonucleotides Genotyping DNA primers (Table S1) This paper N/A siRNA Extl3 MSS225704 UCGUUGUCCU CUGACGUCUGGCUUU Life Technologies Cat# 10620318 siRNA XIAP MSS202115 GCUCACGG AUUGGAAGCCAAGUGAA Life Technologies Cat# 10620318 (Continued on next page) Cell Reports 38, 110462, March 8, 2022 e2

    Bicinchoninic Acid Protein Assay:

    Article Title: Nociceptor-derived Reg3γ prevents endotoxic death by targeting kynurenine pathway in microglia.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER CD11c MicroBeads Myltenyi Biotec Cat# 130-108-338 Mouse TNF-a ELISA kit R&D systems Cat# DY410-05 Mouse IL-6 ELISA kit R&D systems Cat# M6000B Mouse IL-1b ELISA kit R&D systems Cat# DY401 Mouse IL-12 ELISA kit R&D systems Cat# M1270 Mouse Alanine Aminotransferase ELISA Kit MyBiosource Cat# MBS264717 Creatinine Colorimetric Assay Kit Cayman Cat# 700460 Mouse Troponin I, cardiac muscle ELISA Kit Signalway Antibody Cat# EK1821 Mouse Reg3g ELISA kit LSBio Cat# LS-F6890 ATPlite PerkinElmer Cat# 6016493 Glucose 6 Phosphate Assay Kit abcam Cat# ab107923 Phosphoenolpyruvate Assay Kit Abnova Cat# KA3745 Kynurenine / Tryptophan ratio ELISA pack ImmuSmol Cat# ISE-2227 Kynurenine ELISA Kit BioVision Cat# E4629 Quinolinic acid ELISA ImmuSmol Cat# IS I-0100 Phospho-c-Src + Total In-Cell ELISA kit abcam Cat# ab207461 Rac1 G-LISA Activation Assay Kit Cytoskeleton Cat# BK128 ReverTra Ace Toyobo Cat# FSQ-201 Taqman Ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 HMT internal standards Human Metabolome Technologies Cat# H3304-1002 Avidin/biotin blocking kit Vector Cat# SP-2001 BCA protein assay kit TaKaRa Cat# T9300A Western Lightning Plus-ECL reagents Parkin Elmer Cat# NEL103001EA Low Salt Immune Complex Wash Buffer Millipore Cat# 20154 High Salt Immune Complex Wash Buffer Millipore Cat# 20155 LiCl Immune Complex Wash Buffer Millipore Cat# 20156 RNAeasy Mini Kit Qiagen Cat# 74104 Deposited data Microarray data of brain GEO database https://www.ncbi. nlm.gov/geo/ Accession number: GEO: GSE173216 Experimental models: Cell lines Cell line: Ra2 COSMO BIO COS-NMG-RA2C Cell line: N2A ATCC ATCC CCL-131 Cell line: RAW264.7 ATCC ATCC TIB-72 Experimental models: Organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Nav1.8-Cre EMMA Stock ID: EM:04582 Mouse: Rosa26-DTA Jackson Laboratory Stock number: 010527 Mouse: Bcl10-/- Provided by S. Akira Ruefli-Brasse et al. (2003) Mouse: TLR4-/- Purchased from OrientalBioService Hoshino et al. (1999) Mouse: TRPA1-/- Provided by D. Julius Bautista et al. (2006) Mouse: FLPo-Cre Provided by M. Ikawa Yamazaki et al. (2016) Mouse: Reg3gtm1a(KOMP) KOMP repository Strain ID: Reg3 g tm1a (KOMP) Mouse: Villin-Cre Provided by S. Akira el Marjou et al. (2004) Oligonucleotides Genotyping DNA primers (Table S1) This paper N/A siRNA Extl3 MSS225704 UCGUUGUCCU CUGACGUCUGGCUUU Life Technologies Cat# 10620318 siRNA XIAP MSS202115 GCUCACGG AUUGGAAGCCAAGUGAA Life Technologies Cat# 10620318 (Continued on next page) Cell Reports 38, 110462, March 8, 2022 e2

    Western Blot:

    Article Title: Nociceptor-derived Reg3γ prevents endotoxic death by targeting kynurenine pathway in microglia.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER CD11c MicroBeads Myltenyi Biotec Cat# 130-108-338 Mouse TNF-a ELISA kit R&D systems Cat# DY410-05 Mouse IL-6 ELISA kit R&D systems Cat# M6000B Mouse IL-1b ELISA kit R&D systems Cat# DY401 Mouse IL-12 ELISA kit R&D systems Cat# M1270 Mouse Alanine Aminotransferase ELISA Kit MyBiosource Cat# MBS264717 Creatinine Colorimetric Assay Kit Cayman Cat# 700460 Mouse Troponin I, cardiac muscle ELISA Kit Signalway Antibody Cat# EK1821 Mouse Reg3g ELISA kit LSBio Cat# LS-F6890 ATPlite PerkinElmer Cat# 6016493 Glucose 6 Phosphate Assay Kit abcam Cat# ab107923 Phosphoenolpyruvate Assay Kit Abnova Cat# KA3745 Kynurenine / Tryptophan ratio ELISA pack ImmuSmol Cat# ISE-2227 Kynurenine ELISA Kit BioVision Cat# E4629 Quinolinic acid ELISA ImmuSmol Cat# IS I-0100 Phospho-c-Src + Total In-Cell ELISA kit abcam Cat# ab207461 Rac1 G-LISA Activation Assay Kit Cytoskeleton Cat# BK128 ReverTra Ace Toyobo Cat# FSQ-201 Taqman Ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 HMT internal standards Human Metabolome Technologies Cat# H3304-1002 Avidin/biotin blocking kit Vector Cat# SP-2001 BCA protein assay kit TaKaRa Cat# T9300A Western Lightning Plus-ECL reagents Parkin Elmer Cat# NEL103001EA Low Salt Immune Complex Wash Buffer Millipore Cat# 20154 High Salt Immune Complex Wash Buffer Millipore Cat# 20155 LiCl Immune Complex Wash Buffer Millipore Cat# 20156 RNAeasy Mini Kit Qiagen Cat# 74104 Deposited data Microarray data of brain GEO database https://www.ncbi. nlm.gov/geo/ Accession number: GEO: GSE173216 Experimental models: Cell lines Cell line: Ra2 COSMO BIO COS-NMG-RA2C Cell line: N2A ATCC ATCC CCL-131 Cell line: RAW264.7 ATCC ATCC TIB-72 Experimental models: Organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Nav1.8-Cre EMMA Stock ID: EM:04582 Mouse: Rosa26-DTA Jackson Laboratory Stock number: 010527 Mouse: Bcl10-/- Provided by S. Akira Ruefli-Brasse et al. (2003) Mouse: TLR4-/- Purchased from OrientalBioService Hoshino et al. (1999) Mouse: TRPA1-/- Provided by D. Julius Bautista et al. (2006) Mouse: FLPo-Cre Provided by M. Ikawa Yamazaki et al. (2016) Mouse: Reg3gtm1a(KOMP) KOMP repository Strain ID: Reg3 g tm1a (KOMP) Mouse: Villin-Cre Provided by S. Akira el Marjou et al. (2004) Oligonucleotides Genotyping DNA primers (Table S1) This paper N/A siRNA Extl3 MSS225704 UCGUUGUCCU CUGACGUCUGGCUUU Life Technologies Cat# 10620318 siRNA XIAP MSS202115 GCUCACGG AUUGGAAGCCAAGUGAA Life Technologies Cat# 10620318 (Continued on next page) Cell Reports 38, 110462, March 8, 2022 e2

    Microarray:

    Article Title: Nociceptor-derived Reg3γ prevents endotoxic death by targeting kynurenine pathway in microglia.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER CD11c MicroBeads Myltenyi Biotec Cat# 130-108-338 Mouse TNF-a ELISA kit R&D systems Cat# DY410-05 Mouse IL-6 ELISA kit R&D systems Cat# M6000B Mouse IL-1b ELISA kit R&D systems Cat# DY401 Mouse IL-12 ELISA kit R&D systems Cat# M1270 Mouse Alanine Aminotransferase ELISA Kit MyBiosource Cat# MBS264717 Creatinine Colorimetric Assay Kit Cayman Cat# 700460 Mouse Troponin I, cardiac muscle ELISA Kit Signalway Antibody Cat# EK1821 Mouse Reg3g ELISA kit LSBio Cat# LS-F6890 ATPlite PerkinElmer Cat# 6016493 Glucose 6 Phosphate Assay Kit abcam Cat# ab107923 Phosphoenolpyruvate Assay Kit Abnova Cat# KA3745 Kynurenine / Tryptophan ratio ELISA pack ImmuSmol Cat# ISE-2227 Kynurenine ELISA Kit BioVision Cat# E4629 Quinolinic acid ELISA ImmuSmol Cat# IS I-0100 Phospho-c-Src + Total In-Cell ELISA kit abcam Cat# ab207461 Rac1 G-LISA Activation Assay Kit Cytoskeleton Cat# BK128 ReverTra Ace Toyobo Cat# FSQ-201 Taqman Ribosomal RNA control reagents Thermo Fisher Scientific Cat# 4308329 HMT internal standards Human Metabolome Technologies Cat# H3304-1002 Avidin/biotin blocking kit Vector Cat# SP-2001 BCA protein assay kit TaKaRa Cat# T9300A Western Lightning Plus-ECL reagents Parkin Elmer Cat# NEL103001EA Low Salt Immune Complex Wash Buffer Millipore Cat# 20154 High Salt Immune Complex Wash Buffer Millipore Cat# 20155 LiCl Immune Complex Wash Buffer Millipore Cat# 20156 RNAeasy Mini Kit Qiagen Cat# 74104 Deposited data Microarray data of brain GEO database https://www.ncbi. nlm.gov/geo/ Accession number: GEO: GSE173216 Experimental models: Cell lines Cell line: Ra2 COSMO BIO COS-NMG-RA2C Cell line: N2A ATCC ATCC CCL-131 Cell line: RAW264.7 ATCC ATCC TIB-72 Experimental models: Organisms/strains Mouse: C57BL/6J Japan CLEA C57BL/6JJcl Mouse: Nav1.8-Cre EMMA Stock ID: EM:04582 Mouse: Rosa26-DTA Jackson Laboratory Stock number: 010527 Mouse: Bcl10-/- Provided by S. Akira Ruefli-Brasse et al. (2003) Mouse: TLR4-/- Purchased from OrientalBioService Hoshino et al. (1999) Mouse: TRPA1-/- Provided by D. Julius Bautista et al. (2006) Mouse: FLPo-Cre Provided by M. Ikawa Yamazaki et al. (2016) Mouse: Reg3gtm1a(KOMP) KOMP repository Strain ID: Reg3 g tm1a (KOMP) Mouse: Villin-Cre Provided by S. Akira el Marjou et al. (2004) Oligonucleotides Genotyping DNA primers (Table S1) This paper N/A siRNA Extl3 MSS225704 UCGUUGUCCU CUGACGUCUGGCUUU Life Technologies Cat# 10620318 siRNA XIAP MSS202115 GCUCACGG AUUGGAAGCCAAGUGAA Life Technologies Cat# 10620318 (Continued on next page) Cell Reports 38, 110462, March 8, 2022 e2



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